Synopsis: Make No Assumptions

A new method of imaging with second harmonic light allows more general studies of molecules with different orientations.
Synopsis figure
J. Duboisset et al., Phys. Rev. A (2012)

Second Harmonic Generation (SHG) microscopy is a widely used technique to image biomolecular assemblies in complex samples. The technique measures the second harmonic of light emitted by a molecule in the presence of an intense polarized light source and is sensitive to a molecule’s orientation because the emitted light depends on the angle between the incident light’s polarization and the molecule. Writing in Physical Review A, Julien Duboisset and colleagues at the Fresnel Institute in Marseilles, France, propose, and experimentally test, a method that circumvents a previous limitation of the polarized SHG microscopy technique.

Polarized SHG microscopy has been used to deduce the molecular orientation of molecular materials, collagen, and muscle structure in tissues. However, to date, gleaning structural information from the microscopy data relied on assumed models of the molecules’ spatial orientations. Such models typically fail to give a complete picture of the molecular orientation, primarily because there is no way to validate them.

Duboisset et al.’s method is a generic approach that does not rely on a specific model of the orientation of the molecules in the sample and includes possible geometries that were not previously accessible. They applied their method to the imaging of assemblies in isolated collagen fibers, which are a major component of connective tissue. This method of imaging has potential applications for studying both molecular and biological materials. – Frank Narducci


Announcements

More Announcements »

Subject Areas

OpticsBiological Physics

Previous Synopsis

Related Articles

Viewpoint: Squeezed Light Reengineers Resonance Fluorescence
Atomic and Molecular Physics

Viewpoint: Squeezed Light Reengineers Resonance Fluorescence

By bathing a superconducting qubit in squeezed light, researchers have been able to confirm a decades-old prediction for the resulting phase-dependent spectrum of resonance fluorescence. Read More »

Synopsis: Polarons Drive a Magneto-Optical Effect
Magnetism

Synopsis: Polarons Drive a Magneto-Optical Effect

A surprisingly large magneto-optical response occurs when mobile electrons in a cooled material become trapped by their interaction with the surrounding lattice. Read More »

Focus: Biological Cells Form Electric Circuits
Biological Physics

Focus: Biological Cells Form Electric Circuits

Cells that are electrically active and that also produce light for easy voltage monitoring could lead to new studies of heart arrhythmias and possibly bio-computing. Read More »

More Articles